Thymosin Beta-4 Research Reference

Catalog classification, reference sizes, lab math links, and source-backed data. Educational use only — not medical advice.

Researched for: Thymosin Beta-4 evidence and protocol review

Structural Research peptide Lyophilized verified

Also known as: Tbeta4, T4, TB4

Open in Calculator ← Compound Index All Tools

Quick Facts

Reference Sizes

5 mg, 10 mg

Calculator

Preset available

Storage

Lyophilized: store at -20 deg C. After reconstitution: refrigerate 2-8 deg C; verify beyond-use timing against source COA.

Solubility

Form, salt, and formulation dependent; verify the lot-specific COA

Catalog Description

Thymosin beta-4 (Tbeta4) is the naturally occurring 43-amino-acid actin-sequestering protein from which TB-500 research material is derived/modeled. The foundational wound-healing data (Malinda et al., rat full-thickness wound model: 42-61% increased reepithelialization, increased collagen deposition and angiogenesis) applies to full-length Tbeta4. See the TB-500 record for the same core mechanism and citations; this record represents the parent full-length molecule rather than the shorter research fragment sometimes sold as 'TB-500.'

Catalog descriptions reflect research context only. Not a medical claim or treatment recommendation.

Research Profile

Half-Life

No single systemic human half-life established from ophthalmic research

Onset

Rodent models show measurable wound-healing effects within 4-7 days

Administration Routes (Research)

IV in the phase-I healthy-volunteer studyOphthalmic in dry-eye and corneal studies

Reported Research Effects

Accelerated reepithelialization in rodent wound models
Promotes endothelial/keratinocyte/progenitor cell migration via actin sequestration
Angiogenesis via VEGF-pathway involvement

Reported Research Side Effects

Mild to moderate adverse events in the phase-I IV study
No dose-limiting toxicity or serious adverse events reported there
No adverse events in the 72-person phase-II ophthalmic study
Angiogenesis and cell-migration biology require context-specific risk assessment

Data source: Primary literature, regulator records, trial registries, and identity-specific evidence search

Reflects preclinical/research literature context only. Not a medical claim, dosing instruction, or treatment recommendation.

Research Protocols Reported in the Literature

Single IV ascending doses of 0.05, 0.25, 0.5, 2.0, 5.0, 12.5, or 25.0 mcg/kg [IV; First-in-human randomized phase-I study in healthy volunteers; Single dose]Source: PMID 34346165
0.5, 2.0, or 5.0 mcg/kg IV once daily for 10 days [IV; First-in-human multiple-dose phase-I cohorts; Daily for 10 days]Source: PMID 34346165
0.1% thymosin beta-4 ophthalmic solution for 28 days [Ophthalmic; Randomized phase-II dry-eye study; Study schedule not extractable from abstract]Source: PMID 26056426
Sterile thymosin beta-4 eye drops for 28 or 49 days [Ophthalmic; Nine-patient compassionate-use corneal-defect series; Study schedule not extractable from abstract]Source: PMID 20536469

Research Notes

Full-length 43-amino-acid thymosin beta-4 is distinct from the seven-amino-acid TB-500 fragment. The IV phase-I study tested recombinant full-length Tbeta4 and cannot validate systemic TB-500 schedules.

Supplementary research context only. Not medical advice or a treatment recommendation.

Sources

Recombinant human thymosin beta-4 first-in-human phase-I study — PubMed (NIH)
https://pubmed.ncbi.nlm.nih.gov/34346165/

Thymosin beta-4 phase-II dry-eye trial — PubMed (NIH)
https://pubmed.ncbi.nlm.nih.gov/26056426/

Thymosin beta-4 corneal-defect compassionate-use series — PubMed (NIH)
https://pubmed.ncbi.nlm.nih.gov/20536469/

FDA TB-500 briefing distinguishing full-length thymosin beta-4 — U.S. Food and Drug Administration
https://www.fda.gov/media/193349/download

Suggest a community source

Found a relevant Reddit or forum thread about this compound? Suggest it below. Every suggestion is reviewed by an editor before it appears -- nothing is published automatically.

Related Research Tools

Reconstitution CalculatorConcentration, draw volume, per-unit amounts Unit Convertermg, mcg, mL, units conversion Compound CompareSide-by-side comparison tool Cost CalculatorCost per mg, mcg, per-draw

Research Use Only. All content is for educational and research reference only. Not medical advice, a treatment recommendation, or guidance on human use. Compounds are not FDA-approved drugs unless specifically noted.